Addgene: Recipient Instructions. In those cases, we ask that the recipient scientist forward the rsa to their pi. Addgene mails an invoice listing the po number or charges your credit card.
Addgene GLI K12
This plasmid is available through addgene. Pellet contains the precipitated dna. Login to your addgene account. This freezing may help the dna to precipitate. Where can i find a list of all of my. How can i check the status of my order? Transformation, transduction, and conjugation occur in nature as. Invert the microfuge tube to mix. Thank you for helping your researchers get the materials they need! This kit is built for serious multiplexing and enables users to express up to 7 grnas.
After you have bacterial growth, add 500 μl of the overnight culture to 500 μl of 50% glycerol in a 2 ml screw top tube or cryovial and gently mix. Follow the steps for inoculating an overnight liquid culture. The recipient scientist (rs) listed on the order should be the person in the lab who will be receiving and primarily working with the materials. Denes hnisz's lab contains the insert fkbp f36v p2a mruby2. Thank you for helping your researchers get the materials they need! Most items in our repository require the approval of an mta. Addgene processes and ships the order, then emails you the tracking number. Login to your addgene account. If there is a problem, please contact addgene within 60 days of receiving your antibody. A request has been made by recipient scientist name at your institution, recipient name for biological materials from provider name that are stored at addgene. Make the 50% glycerol solution by diluting 100% glycerol in dh20.
